Early human contact and housing for pigs - part 2: resilience to routine husbandry practices.

The ability of pigs to cope with routine farming practices can affect their welfare. This paper is part of a series on early experiences and stress, and reports on the effects of early human contact and housing on the responses of pigs to routine husbandry practices. Using a 2 × 2 factorial design, 48 litters of pigs were raised in either a conventional farrowing crate (FC) or a loose farrowing pen (LP; PigSAFE pen) which was larger, more physically complex and allowed the sow to move freely. Piglets were provided with either routine contact from stockpeople (C), or routine contact plus regular opportunities for positive human contact (+HC) involving 5 min of scratching, patting and stroking imposed to the litter 5 days/week from 0 to 4 weeks of age. At 4 weeks of age, piglets were weaned and re-housed with controlled mixing of litters within treatment. At 4 days of age, after only 3 bouts of the handling treatment, +HC pigs showed less escape behaviour than C pigs after capture by a stockperson for vaccinations and tail docking, and shorter durations of vocalisations throughout the procedures. The  +HC pigs also showed less escape behaviour when captured by a stockperson at 3 weeks of age. The FC pigs showed less escape behaviour than LP pigs after capture by a stockperson at 4 days of age but not at 3 weeks of age. Serum cortisol concentrations were lower in FC pigs than LP pigs 2 h after weaning but not at 49 h after weaning, whereas serum cortisol concentrations were lower in  +HC pigs than C pigs at 49 h after weaning but not at 2 h after weaning. In the period from 0 to 1 h after weaning, C pigs from LP performed the most escape attempts, although escape attempts were rare overall. When being moved out of the home pen by a stockperson at 21 weeks of age, FC pigs showed less baulking than LP pigs, but there were no detected effects of human contact treatment. In conclusion, both housing system and human contact during lactation affected the stress responses of pigs to routine husbandry practices. The +HC and FC pigs appeared to cope better than C and LP pigs, based on lower responses indicative of stress including escape behaviour, vocalisations and cortisol concentrations. These findings are consistent with corresponding reductions in fear that were reported in Part 1 of this series of papers.

Analysis of substantiated welfare investigations in extensive farming systems in Victoria, Australia.

Substantiated incidents of poor welfare affecting cattle, sheep and goats (livestock) in non-dairy extensive farming systems continue to occur. This study sought to describe the common causes of poor welfare of livestock and the associated circumstances, by analysing 39 years of de-identified, livestock welfare investigation records. There were a total of 2179 alleged offenders (AOff), defined as individual/s that had an incident of poor welfare affecting livestock on at least one occasion. Approximately 27% of AOff were found to have poor welfare on more than one occasion. The majority of livestock welfare incidents were associated with neglect, more specifically, inadequate nutrition (56%), treatment (65%) and management/husbandry (83%). Records of malicious acts were rare (1%). In the analysis, cases were allocated to 10 animal welfare severity categories (AWSC) based on the number of incidents and visits, whether the AOff reoffended, or if the incident was ongoing and whether the welfare issue was likely to affect the whole herd. A significantly higher proportion of cases in the most severe AWSC had a failure to shear, mark, dip/drench, draft and wean/cull, were overstocked or were not providing proper and sufficient feed, compared to the least severe AWSC (P ≤ 0.05). Reoffending was significantly more likely when animals were found to be injured/unwell, recumbent, stuck in mud/yard/pen or in poor body condition, or when there was a failure to wean/cull, mark, dip/drench and draft. Some of the issues identified here may be risk factors more commonly identified on farms with poor livestock welfare.

Early human contact and housing for pigs - part 1: responses to humans, novelty and isolation.

The development of fear and stress responses in animals can be influenced by early life experiences, including interactions with humans, maternal care, and the physical surroundings. This paper is the first of three reporting on a large experiment examining the effects of the early housing environment and early positive human contact on stress resilience in pigs. This first paper reports on the responses of pigs to humans, novelty, and social isolation. Using a 2 × 2 factorial design, 48 litters of pigs were reared in either a conventional farrowing crate (FC) where the sow was confined or a loose farrowing pen (LP; PigSAFE pen) which was larger, more physically complex and allowed the sow to move freely throughout the farrowing and lactation period. Piglets were provided with either routine contact from stockpeople (C), or routine contact plus regular opportunities for positive human contact (+HC) involving 5 min of scratching, patting and stroking imposed to the litter 5 days/week from 0-4 weeks of age. The positive handling treatment was highly effective in reducing piglets' fear of humans, based on +HC piglets showing greater approach and less avoidance of an unfamiliar person at 3 weeks of age. There was evidence that this reduction in fear of humans lasted well beyond when the treatment was applied (lactation), with +HC pigs showing greater approach and less avoidance of humans in tests at 6, 9 and 14 weeks of age. The +HC treatment also reduced piglets' fear of a novel object at 3 weeks of age, and for pigs in FC, the cortisol response after social isolation at 7 weeks of age. Rearing in FC compared to LP reduced piglets' fear of novelty at 3 weeks of age, as well as their vocalisations and cortisol response to isolation at 7 weeks of age. The FC pigs showed greater approach and less avoidance of humans compared to LP pigs at 3, 4 and 6 weeks of age, but not at 9 and 14 weeks of age. These results show that positive handling early in life can reduce pigs' fear of humans, fear of novelty and physiological stress response to social isolation. The LP pigs were reared in a more isolated environment with less overall contact with stockpeople and other pigs, which may have increased their fear responses to humans and novel situations, suggesting that different housing systems can modulate these pigs' responses.

Early human contact and housing for pigs - part 3: ability to cope with the environment.

Early experiences can have long-term impacts on stress adaptability. This paper is the last of three in a series on early experiences and stress in pigs, and reports on the effects of early human contact and housing on the ability of pigs to cope with their general environment. Using a 2 × 2 factorial design, 48 litters of pigs were reared in either a farrowing crate (FC) or a loose farrowing pen (LP; PigSAFE pen) which was larger, more physically complex and allowed the sow to move freely. Piglets were provided with either routine contact from stockpeople (C), or routine contact plus regular opportunities for positive human contact (+HC) involving 5 min of scratching, patting and stroking imposed to the litter 5 days/week from 0 to 4 weeks of age. At 4 weeks of age (preweaning), C piglets that were reared in FC had considerably lower concentrations of serum brain-derived neurotrophic factor (BDNF) than piglets from the other treatment combinations. Compared to C pigs, +HC pigs had fewer injuries at 4 weeks of age. There were no clear effects of human contact on BDNF concentrations or injuries after weaning, or on basal cortisol or immunoglobulin-A concentrations, behavioural time budgets, tear staining, growth, or piglet survival. Compared to FC piglets, LP piglets showed more play behaviour and interactions with the dam and less repetitive nosing towards pen mates during lactation. There was no evidence that early housing affected pigs' behavioural time budgets or physiology after weaning. Tear staining severity was greater in LP piglets at 4 weeks of age, but this may have been associated with the higher growth rates of LP piglets preweaning. There was no effect of lactation housing on growth after weaning. Preweaning piglet mortality was higher in the loose system. The findings on BDNF concentrations, injuries and play behaviour suggest improved welfare during the treatment period in +HC and LP piglets compared to C and FC piglets, respectively. These results together with those from the other papers in this series indicate that positive human interaction early in life promotes stress adaptability in pigs. Furthermore, while the farrowing crate environment deprives piglets of opportunities for play behaviour and sow interaction, there was no evidence that rearing in crates negatively affected pig welfare or stress resilience after weaning. Whether these findings are specific to the two housing systems studied here, or can be generalised to other housing designs, warrants further research.

Training of transport drivers improves their handling of pigs during loading for transport to slaughter.

Pig transport drivers' (TDs') handling actions are of great importance as these drivers handle a large number of finishing pigs during stressful situations. Poor handling techniques can have negative consequences for working conditions, pig welfare and meat quality. We studied the effects of a training intervention on Swedish TDs' attitudes towards pigs and their handling actions during loading for transport to slaughter. Twenty TDs working with commercial pig transportation in Sweden were recruited and completed an attitude questionnaire. Ten of them were observed during one loading of pigs before training, and one or two loadings after training (49-265 pigs per loading), and eight of them completed the attitude questionnaire again 50-160 days after training. The cognitive-behavioural training program ProHand Pigs® was adapted and delivered to the ten TDs during a group session, followed by individual meetings. TDs' handling actions were video recorded and summarised as binary variables per 5-s interval. Factor analysis, principal component analysis and paired t-test were conducted to investigate the effects of the training intervention on TDs' attitudes, and mixed-effects logistic models were used to examine effects on negative and positive handling actions. Training tended to decrease TDs' beliefs that it is important to move pigs quickly (P = 0.095). Training decreased the odds of a 'moderately to strongly negative' action by 55% (P = 0.0013) and increased the odds of a 'positive' action by 97% (P < 0.0001). This study provides valuable insights into the possibilities to improve TDs' handling actions, and implications for pig welfare during slaughter transport. The study supports previous findings that the attitudes and behaviour of handlers can be improved by cognitive-behavioural training. While our previous research has shown reciprocal relationships between TDs' actions and pig behaviour, further research on TDs' attitudes towards handling that underlie the nature of their behaviour when handling pigs is necessary to fine-tune the cognitive-behavioural training program applied in the present study.

Cystoscopic application of PuraStat® in the treatment of radiation-induced haemorrhagic cystitis.

The use of radiotherapy has increased in recent years, especially for pelvic neoplasms, and this can result in long-term complications such as recurrent haemorrhagic radiation cystitis (RHC). A 73-year-old male patient presented to a hospital emergency department multiple times with visible haematuria and clots leading to urinary clot retention; he was finally diagnosed with RHC. During the last presentation, the bladder was irrigated continuously with saline using a three-way catheter. During hospitalisation, a cystourethroscopy was performed for bladder evaluation and clot evacuation. Multiple bleeding ulcers were recognised on the bladder wall, biopsies were taken for histopathology, and the ulcers cauterised. Packed red blood cell transfusions were required, and sodium hyaluronate (CystiStat®) bladder instillations were tried. There was no clinical improvement following any of these interventions. In light of the patient's deteriorating condition, cystoscopic application of PuraStat® 3ml was administered, which led to remission of the urinary bleeding in the short term. We continue to monitor the effects in the medium and long term. Based on current data, PuraStat® haemostatic agent therapy may be considered for RHC, when traditional treatments are ineffective or infeasible, potentially eliminating the need for more aggressive therapy such as cystectomy.

Review: Early life piglet experiences and impacts on immediate and longer-term adaptability.

Pigs in production systems are routinely exposed to challenging situations including abrupt weaning, painful husbandry procedures, intense contact with stockpeople, and exposure to novel social and physical environments. The resilience of pigs to these stressors has implications for animal welfare and productivity and can be affected by early life experiences. In rodents and primates, early experiences with stressors that the animal can adequately cope with confers future stress adaptability, leading to less abnormal behaviour, lower behavioural and physiological responses to stressors, and faster recovery after stress exposure. Early experiences that can affect the ability of pigs to overcome challenge include interactions with the dam, conspecifics, humans, and the overall complexity of the environment. Farrowing crates limit the sow's ability to show maternal behaviour towards piglets, and negatively affect piglet social behaviour during lactation, with less play and more manipulation of pen mates in crates than in large pens. Rearing in pens has been proposed to improve the ability of pigs to cope with routine stressors, but the evidence for this is conflicting. The early housing environment can affect general fearfulness and fear of humans, and surprisingly, most studies have shown fear responses to be greater in pigs reared pens than in crates. Given the potential for fear to affect animal welfare and productivity, more detailed research on early housing effects is needed. While there is limited evidence that early housing influences fear in the longer term, human contact early in life appears to have a more profound and sustained effect, with regular positive human interaction early in life having an enduring effect on reducing pigs' fear of humans. The practicality of positive human-pig interaction in a commercial environment needs to be examined further, but only a small amount of positive human contact early in life can improve the resilience of pigs to routine husbandry stressors. Early social experience with non-littermates reduces stress at weaning and mixing, while early weaning before 3-4 weeks of age increases abnormal behaviours. Environmental enrichment, such as foraging substrates and increased floor space, reduces abnormal behaviour in piglets, but housing in an enriched environment early in life and subsequently in a non-enriched environment can increase abnormal behaviour if these environments are dramatically different. Although the later environment can modify the influence of the early environment, overall, early life experiences can be important in shaping how pigs cope with stress in both an immediate and longer-term capacity.

Reducing the rate of negative ureteroscopy: predictive factors and the role of preoperative imaging.

INTRODUCTION: The aim of this study was to investigate factors that may predict a negative ureteroscopy (URS) performed for ureteric calculi in prestented patients and to assess preoperative imaging in reducing the rate of negative URS. METHODS: Data were collected on emergency stent placement for a ureteric calculus from April 2011 to February 2016 (Group A) and October 2016 to October 2019 (Group B). Data included patient demographics, indication for a stent, stone characteristics, baseline bloods, urine culture, readmission, negative URS rate and the use of pre-URS imaging. Multivariate logistic regression was used for statistical analysis. RESULTS: Of 257 patients who underwent emergency stent insertion, 251 underwent deferred URS for a ureteric calculus and 6 avoided URS due to pre-URS imaging. Indications for stent were pain (42%), sepsis (39%) and acute kidney injury (19%). Mean stone size was 7.8mm, mean stone density was 699 Hounsfield units (HU) and the stone locations were upper (62%), mid (13%) and lower ureter (25%). The overall negative URS rate was 12%. The negative URS rate was lower in patients with pre-URS imaging compared with those with none, 6% and 14%, respectively (OR=2.33, 95% CI: 0.69-7.56, p=0.2214). Logistic regression analysis indicated stone size as the only significant predictor of a negative URS, where the greater the size of the stone the less likely URS would be negative (ß=0.75, 95% CI: 0.60-0.94 p=0.011). CONCLUSIONS: Utilising pre-URS imaging can lead to a reduction in negative URS rate. Stone size <5mm appears to be the subgroup most likely to benefit from imaging.